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HOME > Gateway Binary Vector (pGWB)
 
Information of Gateway Binary Vector (pGWB)
 
Gateway Vector for BiFC >>     Gateway Recycling Cloning >>     Dual-Site Gateway Cloning >>
 
Review (INTECH Open Access): Gateway Vectors for Plant Genetic Engineering:
Overview of Plant Vectors, Application for Bimolecular Fluorescence
Complementation (BiFC) and Multigene Construction
 

attR1-attR2 type

pGWB series (pBI backbone, Kmr and Hygr for bacteria and plant)
pGWB series
 
Complete List of pGWB PDF download
  • pGWB1-45 (pBI backbone, PNOS:NTPII and P35S:HPT) (List)
  • pGWB203-235 (pBI backbone, PNOS:NTPII and PNOS:HPT) (List)
  • manual Japanese (Word / PDF) manuals  Word / PDF
  • manual English (Word / PDF) manuals  Word / PDF
  • Reference    Most Cited Journal of JBB (last five years)


  • attR1-attR2 type

    ImpGWB series (pPZP backbone, SpcR for bacteria, KmR or HygR or BASTA-R or TunicamycinR for plant)
    ImpGWB series
     
    Complete List of ImpGWB PDF download
  • pGWB401-461 (KmR, pPZP backbone, PNOS:NTPII) (List)
  • Reference
  • pGWB401-455 are available from Addgene

  • pGWB501-561 (HygR, pPZP backbone, PNOS:HPT) (List)
  • Reference
  • pGWB501-555 are available from Addgene

  • pGWB601-661 (BASTA-R, pPZP backbone, PNOS:bar) (List)
  • Reference
  • pGWB601-661 are available from RIKEN BRC

  • pGWB701-761 (TunicamycinR, pPZP backbone, PNOS:GPT) (List)
  • Reference
  • Comments on tunicamycin selection (important)

  • manual Japanese (PDF)
  • manual English (PDF)


  • attR4-attR2 type (Promoter Swapping)

    R4pGWB series (pPZP backbone, SpcR for bacteria, KmR or HygR or BASTA-R or TunicamycinR for plant)
    R4pGWB series
     
    Complete List of R4pGWB PDF download
  • R4pGWB401-459 (KmR, pPZP backbone, PNOS:NTPII) (List)
  • Reference

  • R4pGWB501-559 (HygR, pPZP backbone, PNOS:HPT) (List)
  • Reference

  • R4pGWB601-659 (BASTA-R, pPZP backbone, PNOS:bar) (List)
  • Reference
  • R4pGWB601-659 are available from RIKEN BRC

  • R4pGWB701-759 (TunicamycinR, pPZP backbone, PNOS:GPT) (List)
  • Reference
  • Comments on tunicamycin selection (important)

  • manual Japanese (PDF)
  • manual English (PDF)


  • attR4-attL1type (Promoter Analysis)

    R4L1pGWB series (pPZP backbone, SpcR for bacteria, KmR or HygR or BASTA-R for plant)
    R4L1pGWB series
     
    Complete List of R4L1pGWB PDF download
  • R4L1pGWB432-459 (KmR, pPZP backbone, PNOS:NTPII) (List)
  • Reference

  • R4L1pGWB532-559 (HygR, pPZP backbone, PNOS:HPT) (List)
  • Reference

  • R4L1pGWB632-659 (BASTA-R, pPZP backbone, PNOS:bar) (List)
  • Reference
  • MTA for BASTA-R R4L1 vectors Japanese (Word / PDF)
  • MTA for BASTA-R R4L1 vectors English (Word / PDF)

  • R4L1pGWB732-759 (TunicamysinR, pPZP backbone, PNOS:GPT (List)
  • Reference
  • Comments on tunicamycin selection (important)

  • manual Japanese (PDF)
  • manual English (PDF)


  • Gateway Vector for BiFC (not binary)

    Gateway Vector for BiFC (pUC119 backbone, Ampr for bacteria)
    Gateway Vector for BiFC
     
    Complete List of Gateway Vector for BiFC PDF download
  • pGWnY (nYFP/pUGW2) (List)
  • pnYGW (nYFP/pUGW0)
  • pGWcY (cYFP/pUGW2)
  • pcYGW (cYFP/pUGW0)
  • Reference
  • Reference for former name


  • Gateway Recycling Cloning Ver. 1
    recycle illustration


    Gateway Vector for Recycling Cloning Ver. 1 (pDONR201 backbone, Kmr for bacteria)
    Gateway Recycling Cloning
     
    Complete List of Gateway Vector for Recycling Cloning Ver. 1 PDF download
  • pRED419 (List)
  • pRED418
  • pCON
  • Reference

    NOTE    Multiple use of CaMV35S promoters frequently causes silencing in this system.


  • Gateway Recycling Cloning Ver. 2

    Gateway Vector for Recycling Cloning Ver. 2 (pDONR201 backbone, Kmr for bacteria)
    Gateway Recycling Cloning
     
    Improvements in Ver.2
    (1) Single digestion at RCS prior to LR reaction is enough for Ver.2 (Double digestion at RCS is necessary for Ver. 1).
    (2) Ampicillin selection is available in transformation after LR reaction using pRED419-Amp or pRED418-Amp.
    (3) Chloramphenicol selection is available in transformation after LR reaction using pCON-Cm-rare1 or pCON-Cm-rare2.
    (4) Use of ampicillin (chloramphenicol) greatly enhances selection of correctly constructed clones in transformation.

    Complete List of Gateway Vector for Recycling Cloning Ver. 2 PDF download
  • pRED419-Amp (List)
  • pRED418-Amp
  • pCON-Cm-rare1
  • pCON-Cm-rare2
  • Reference In preparation

    NOTE    Multiple use of CaMV35S promoters frequently causes silencing in this system.


  • R4 Dual-Site (R4DS) Gateway Cloning System

    Cloning of two set of promoter and ORF into a binary vector.
    R4pGWB6xxx-MD8 series (pPZP backbone. SpcR for bacteria. KmR or HygR or BASTA-R or TunicamycinR for plant)
    [Intermediate vector R4pDD6xx-MD8 series (pUC119 backbone. AmpR for bacteria)
    R4DS illust
     
    Complete List of R4DS Gateway Cloning System PDF download
  • R4pGWB6401-MD8 to R4pGWB6459-MD8, R4pGWB6400-MD8-n/cYFP (KmR, pPZP backbone, Pos:NPTII) (List)
  • Reference

  • R4pGWB6501-MD8 to R4pGWB6559-MD8, R4pGWB6500-MD8-n/cYFP (HygR, pPZP backbone, Pos:HPT) (List)
  • Reference

  • R4pGWB6601-MD8 to R4pGWB6659-MD8, R4pGWB6600-MD8-n/cYFP (BASTA-R, pPZP backbone, Pos:bar) (List)
  • Reference

  • R4pGWB6701-MD8 to R4pGWB6759-MD8, R4pGWB6700-MD8-n/cYFP (KmR, pPZP backbone, Pos:NPTII) (List)
  • Reference

  • R4pDD601-MD8 to R4pDD659-MD8, R4pDD600-MD8-n/cYFP (AmpR, pUC119 backbone) (List)
  • Reference

    All vectors of R4DS Gateway Cloning System are available from RIKEN BRC.
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